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What are you measuring?

In Svalbard I will be responsible for measuring chlorophyll a content. Furthermore, I will take samples for HPLC (High Performance Liquid Chromatography) with which you are able to determine various accessory algae pigments in addition to chlorophyll a. Since different types of algae contain different accessory pigments, the HPLC samples may allow us to see CO2 related changes in the phytoplankton community

What techniques/instruments are you using?

Water samples will be taken from the nine CO2-mesocosms and in these water samples chlorophyll a and additional algae pigments (measured with HPLC) are measured after filtration of a certain water volume on to a filter. The filters are subsequently frozen until the day of measurement. The chlorophyll a content will be measured on site, while the HPLC samples will be carried out, when we get back to Kiel.

How frequently are you measuring samples?

Water samples for determination of chlorophyll a and accessory algae pigments will be taken daily. In addition to water samples, sediment trap samples will be collected for determination of algae pigments.

Why is this important?

Measurements of chlorophyll a are used to determine differences in the biomass of the phytoplankton between the CO2-mesocosms – and thus to see how CO2 affects the amount of chlorophyll a. The HPLC measurements of the accessory algae pigments can eventually be used to characterize the phytoplankton community and how it changes over time within and between CO2-mesocosms.

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